Muni's 44-O'Shaughnessy bus stops near the front of the museum on Tea Garden Drive (southbound) and on Concourse Drive near the Academy of Sciences (northbound).
The 5-Fulton stops at 8th Avenue and Fulton Street, just outside of the park. From 8th, walk into the park and turn right on John F. Kennedy Drive to reach the museum's tower entrance.
The N-Judah Muni Metro streetcar, which is accessible, stops at 9th Avenue and Irving Street. To reach the de Young, walk north on 9th Avenue to Golden Gate Park. 9th Avenue turns into Martin Luther King, Jr. Boulevard. Keep walking on Martin Luther King, Jr. Boulevard until you reach Hagiwara Tea Garden Drive, then turn right and continue to the museum entrance.